The Caniine RLN Test is based on sandwich lateral flow immunochromatographic assay. The test device has a testing window for the observation of assay running and result reading. The testing window has an invisible T (test) zone and a C (control) zone before running the assay. When the treated sample was applied into the sample hole on the device, the liquid will laterally flow through the surface of the test strip and react with the pre-coated monoclonal antibodies. If the concentration of Relaxin in the specimen is exceeding the detection limit, a visible T line will appear. The C line should always appear after a sample is applied, which indicates a valid result. By this means, the device can indicate the presence of relaxin above the detection limit in the specimen.